كيمياء تحليلية Introduction to Modern Liquid Chromatography, Third Edition Lloyd R. Snyder, Joseph J. Kirkland, Joh

المصدر: Introduction to Modern Liquid Chromatography, Third Edition Lloyd R. Snyder, Joseph J. Kirkland, Joh
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B American Society for Mass Spectrometry, 2011 It would be difficult to overstate the importance of liquidchromatography-​

based separations for the preparation of​

samples for mass spectrometry (MS) analysis. Thus, knowledge​

of liquid chromatography (LC) methods and instrumentation is​

often an essential part of an MS user’s education. The book​

Introduction to Modern Liquid Chromatography, while not​

written specifically for the mass spectrometry community,​

contains a wealth of knowledge that is useful for anyone using​

LC. The authors, Lloyd R. Snyder, Joseph J. Kirkland, and John​

W. Dolan, are early pioneers in the development of highperformance​

liquid chromatography (HPLC). Their knowledge​

of the field and years of experience teaching it to others is clearly​

evident in the organization and presentation of the material.​

Additionally, there are eight additional authors who made​

significant contributions to specific chapters or parts of chapters.​

The work is more comprehensive than one would expect for an​

introductory text. Each section is clearly presented with numerous​

cross-references, making it easy for a reader to find those sections​

of particular interest. At the same time, there is enough duplication​

of concepts and even illustrative figures that each section can be​

understood without the need to refer to other sections. Another​

feature worthy of note is that sections that are likely to be ofinterest to only a few readers are clearly marked. The work is​

current, with many references to papers published in 2009.​

The material is organized in straightforward manner. The​

early chapters (1–9) provide the core knowledge that every​

chromatographer should have. Chapter 1 gives a short​

history of HPLC, where it stands in relation to other​

separation techniques, and where additional information on​

the subject can be found. Chapter 2 covers the basic aspects​

of the chromatographic process and method development.​

Chapter 3 covers the hardware components of an HPLC​

system, except for detectors and columns, which are​

described in Chaps. 4 and 5. Emphasis is on understanding​

operational principals with little or no reference to specific​

products or companies. Two chapters are devoted to the​

reverse-phase chromatography of neutral samples (Chap. 6)​

and ionic samples (Chap. 7), and a single chapter (Chap. 8)​

is devoted to normal-phase LC separations. Finally, the​

principles of gradient elution are covered in Chap. 9.​

The later chapters (10–17) are devoted to more advanced​

topics, including: computer-assisted method development​

(Chap. 10); qualitative and quantitative analysis (Chap. 11);​

method validation (Chap. 12); biochemical and synthetic​

polymer separations (Chap. 13); enantiomer separations (Chap.​

14); preparative separations (Chap. 15); sample preparation​

(Chap. 16); and a very useful chapter on troubleshooting​

techniques (Chap. 17). Additionally, Appendix 1 provides​

useful information on the properties of commonly used HPLC​

solvents, and Appendix 2 provides recipes for commonly used​

buffer systems. Each topic is covered in sufficient detail with​

enough literature references that one can become very quickly​

oriented with regard to a particular technique or class of​

compounds.​

In summary, I would strongly recommend this book for​

anyone working with LC and LC/MS. It is a finely crafted​

introduction that adequately covers nearly every aspect of​

the science. The emphasis on basic principles and practical​

aspects ensures that it will be a useful reference for many​

years to come. الرجاء المساعدة في الحصول على هذا المرجع​