almoneer
Active Member
B American Society for Mass Spectrometry, 2011 It would be difficult to overstate the importance of liquidchromatography-
based separations for the preparation of
samples for mass spectrometry (MS) analysis. Thus, knowledge
of liquid chromatography (LC) methods and instrumentation is
often an essential part of an MS user’s education. The book
Introduction to Modern Liquid Chromatography, while not
written specifically for the mass spectrometry community,
contains a wealth of knowledge that is useful for anyone using
LC. The authors, Lloyd R. Snyder, Joseph J. Kirkland, and John
W. Dolan, are early pioneers in the development of highperformance
liquid chromatography (HPLC). Their knowledge
of the field and years of experience teaching it to others is clearly
evident in the organization and presentation of the material.
Additionally, there are eight additional authors who made
significant contributions to specific chapters or parts of chapters.
The work is more comprehensive than one would expect for an
introductory text. Each section is clearly presented with numerous
cross-references, making it easy for a reader to find those sections
of particular interest. At the same time, there is enough duplication
of concepts and even illustrative figures that each section can be
understood without the need to refer to other sections. Another
feature worthy of note is that sections that are likely to be ofinterest to only a few readers are clearly marked. The work is
current, with many references to papers published in 2009.
The material is organized in straightforward manner. The
early chapters (1–9) provide the core knowledge that every
chromatographer should have. Chapter 1 gives a short
history of HPLC, where it stands in relation to other
separation techniques, and where additional information on
the subject can be found. Chapter 2 covers the basic aspects
of the chromatographic process and method development.
Chapter 3 covers the hardware components of an HPLC
system, except for detectors and columns, which are
described in Chaps. 4 and 5. Emphasis is on understanding
operational principals with little or no reference to specific
products or companies. Two chapters are devoted to the
reverse-phase chromatography of neutral samples (Chap. 6)
and ionic samples (Chap. 7), and a single chapter (Chap. 8)
is devoted to normal-phase LC separations. Finally, the
principles of gradient elution are covered in Chap. 9.
The later chapters (10–17) are devoted to more advanced
topics, including: computer-assisted method development
(Chap. 10); qualitative and quantitative analysis (Chap. 11);
method validation (Chap. 12); biochemical and synthetic
polymer separations (Chap. 13); enantiomer separations (Chap.
14); preparative separations (Chap. 15); sample preparation
(Chap. 16); and a very useful chapter on troubleshooting
techniques (Chap. 17). Additionally, Appendix 1 provides
useful information on the properties of commonly used HPLC
solvents, and Appendix 2 provides recipes for commonly used
buffer systems. Each topic is covered in sufficient detail with
enough literature references that one can become very quickly
oriented with regard to a particular technique or class of
compounds.
In summary, I would strongly recommend this book for
anyone working with LC and LC/MS. It is a finely crafted
introduction that adequately covers nearly every aspect of
the science. The emphasis on basic principles and practical
aspects ensures that it will be a useful reference for many
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