المصدر: Collection books of "Methods in Molecular Biology" في منتدى : قسم الأحياء السلام عليكم ورحمة الله وبركاته نقدم اليوم إن شاء الله مجموعة من الاصدارات لسلسلة الكتب Methods in Molecular Biology cell ============== Plant Reverse Genetics: Methods and Protocols Humana Press; 1st Edition | October 11, 2010 | ISBN-10: 1607616815 | 293 pages | PDF | 6.79 MB After the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gene functions need to be widely implemented. In Plant Reverse Genetics: Methods and Protocols, leading researchers in the field describe cutting-edge methods, both high-throughput and genome-wide, involving the models Arabidopsis and rice as well as several other plants to provide comparative functional genomics information. With chapters on the analysis of high-throughput genome sequence data, the identification of non-coding RNA from sequence information, the comprehensive analysis of gene expression by microarrays, and metabolomic analysis, the thorough methods of the book are fully supported by scripts to aid their computational use. ============== Transgenic Plants: Methods and Protocols Humana Press; 1 edition | August 16, 2004 | ISBN-10: 1588292630 | 448 pages | PDF | 8.76 MB A collection of readily reproducible techniques for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems for the regeneration of whole transgenic plants. Among the techniques discussed for the detection of transgenes are quantitative real-time PCR, RT-PCR, in situ hybridization, FISH, and TAIL-PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. ============== Mitochondria: Practical Protocols Humana Press; 1 edition | June 12, 2007 | ISBN-10: 1588296679 | 608 pages | PDF | 8.62 MB This book offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. ============== p53 Protocols Humana Press; 1 edition | June 20, 2003 | ISBN-10: 1588291065 | 296 pages | PDF | 2.38 MB An indispensable collection of novel techniques that have proven most useful for studying the physiological properties of p53 both in vitro and in vivo. The techniques provide proven solutions to problems in studying the purification, target identification, gene expression, quantitation, interaction, signaling, transactivation, and transrepression of p53. The methods are also useful for delineating the functions of other proteins that may act as tumor or growth suppressors. Each technique includes step-by-step instructions, troubleshooting notes, a theoretical review, and discussion of associated problems that might arise during the course of investigation. ============== MAP Kinase Signaling Protocols Humana Press; 1 edition | January 8, 2004 | ISBN-10: 0896039986 | 332 pages | PDF | 4.38 MB An outstanding collection of both classic and cutting-edge techniques for the detection and measurement of mitogen-activated protein kinase (MAPK) signaling cascades. The protocols includes methods for the determination of the subcellular localization of these components, the structural and biophysical analysis of the components, and identification of novel components of known and unknown signaling cascades. Additional methods examine the upstream mechanisms of activation of MAPK cascades by various receptors, the mechanisms involved in the down regulation of the MAPK cascades, and identification of targets of the MAPK cascades. A number of techniques use inhibitors in research on MAPK and transgenic mice for studies of MAPK signaling. The techniques offer biochemists, cell biologists, physicians, and biotechnologists all the essential laboratory techniques needed in to conduct productive studies of MAPK signaling in health and disease, in measuring the influence of drugs, and in a broad range of experimental systems. ============== Gene Probes: Principles and Protocols Humana Press; 1 edition | September 20, 2001 | ISBN-10: 0896038858 | 288 pages | PDF | 1.12 MB Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions. ============== Tumor Suppressor Genes Humana Press; 1 edition | March 3, 2003 | ISBN-10: 0896039862 | 520 pages | PDF | 5.17 MB Leading physician scientists and academic researchers review all the known tumor suppressor genes, explain how they work, and describe how they were discovered and isolated. In many cases, the authors discuss specific genes that are frequently involved in hereditary or sporadic cancers. They also provide a detailed guide to using powerful molecular genetic, cytogenetic, proteomic, and cell biological strategies to discover and isolate novel tumor suppressor genes and their targets. ============== Protein Kinase Protocols Humana Press; 1st edition | January 15, 2001 | ISBN-10: 0896037002 | 365 pages | PDF | 2.84 MB In Protein Kinase Protocols, a panel of highly skilled laboratory investigators describe both basic and more sophisticated methods for the analysis of kinase-mediated signaling cascades, with emphasis on the identification of proteins according to their interactive relationships and the analysis of their functional properties. Described in step-by-step detail, these readily reproducible techniques offer novices quick access to a complicated field, and more experienced investigators many novel and time-saving ploys. Emphasis is given to critical technical steps that are often omitted from methods published in the primary literature. Broad in its range of techniques and thoroughly detailed to help ensure experimental success, Protein Kinase Protocols offers both novice and experienced investigators powerful tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets. ============== Inositol Phosphates and Lipids: Methods and Protocols Humana Press; 1st Edition | May 27, 2010 | ISBN-10: 1603271740 | 240 pages | PDF | 4.6 Mb Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls. ============== Membrane Transporters: Methods and Protocols Humana Press; 1 edition | May 2, 2003 | ISBN-10: 1588291049 | 384 pages | PDF | 3.61 MB This collection of cutting-edge methodologies for studying membrane transporters and channels takes advantage of all the latest developments in biomedical research, including pharmacogenomcs, bioinformatics, and microarray technologies. The authors explain databases and tools for bioinformatics studies, provide practical guidelines for microarray experiments and data analysis, and illustrate the use of small angle X-ray scattering, nuclear magnetic resonance (NMR), and molecular modeling to study the structural biology of membrane transporters. Methods for exploring structure-function correlation, such as site-directed mutagenesis, immunocytochemistry, and confocal microcopy are also described, along with several that may help in the development of novel therapeutics. ============== Molecular and Cell Biology Methods for Fungi Publisher: Humana Press | ISBN: 1607616106 | edition 2010 | PDF | 290 pages | 8,6 mb With the development of fungal transformation systems and the deciphering of an increasing number of fungal genomes, this diverse clade of heterotrophic eukaryotic organisms has proven to be ideal for molecular work and highly serviceable as model systems to study basic processes with results that are applicable to many organisms, including humans. In Molecular and Cell Biology Methods for Fungi, experts in the field provide an up-to-date set of practical protocols covering a range of frequently used methods used to study molecular and cellular aspects of fungal biology. The included classical protocols such as transformation systems and traditional protein analysis methods, which have been widely used for many years, alongside the most advanced techniques such as genome amplification, whole genome knockoutmethods , and sophisticated in vivo imaging techniques will prove to be easily adaptable and useful in a wide range of species. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. ============== Immunoelectron Microscopy: Methods and Protocols Humana Press | 2005 | ISBN: 160761782X | 362 pages | PDF | 5 MB Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. . Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships. ============== KaCarbon Nanotubes: Methods and Protocols Humana Press | 2010 | ISBN: 1607615770 | 240 pages | PDF | 18,2 MB Due to their rare combination of high chemical stability, exceptional optical and electrical properties, high surface-to-volume ratio, and high aspect ratio, carbon nanotubes (CNTs) have made an enormous impact on materials science, molecular biology, biomedicine, and bioanalytical chemistry. Carbon Nanotubes: Methods and Protocols provides reliable, consistent protocols on the application of CNTs in molecular biology-related fields. These are of vital importance, as the commercially available CNTs differ in purity, agglomeration state, as well as length and diameter distribution, all of which have a profound influence on the dispersability and surface properties of the tubes. . The volume contains detailed sections on functionalization, toxicity, trafficking, scaffolds, and biosensors, provided by expert researchers from various fields. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Carbon Nanotubes: Methods and Protocols serves to contribute to the achievement of common standards and helps researchers to avoid discrepancies in future biology-related CNT studies. ============== نسألكم الدعاء وبالتوفيق للجميع Biopolymer Methods in Tissue Engineering Publisher: Humana Press | ISBN: 0896039676 | edition 2003 | PDF | 255 pages | 5 mb Expert laboratory researchers describe in a standard format all the diverse laboratory methods needed to perform state-of-the-art tissue engineering. Topics range from the synthesis, processing, and characterization of specific biomaterials, through the successful use of scaffolds in the engineering of tissues, to techniques useful in evaluating the biological quality of scaffold-engineered tissues. Topics of special interest include the incorporation of biological molecules into scaffold biomaterials and the use of a wide range of methods and techniques to generate a comprehensive description of cell-polymer construct quality. ============== Functional Genomics Publisher: Humana Press | ISBN: 1617374741 | edition 2010 | PDF | 250 pages | 5,1 mb This collection of robust, readily reproducible methods for microarray-based studies includes expert guidance in the optimal data analysis and informatics. On the methods side are proven techniques for monitoring subcellular RNA localization en masse, for mapping chromosomes at the resolution of a single gene, and for surveying the steady-state genome-wide distribution of DNA binding proteins in vivo. For those workers dealing with massive data sets, the book discusses the methodological aspects of data analysis and informatics in the design of microarray experiments, the choice of test statistic, and the assessment of observational significance, data reduction, and clustering. ============== Mycobacteria Protocols Humana Press; 2nd edition | October 20, 2008 | ISBN-10: 1588298892 | 475 pages | PDF | 6.31 MB Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need. Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field. Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular Biology™ series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Capillary Electrophoresis of Proteins and Peptides Humana Press; 1 edition | June 3, 2004 | ISBN-10: 1588290174 | 344 pages | PDF | 7.94 MB Expert academic and pharmaceutical researchers describe their best capillary electrophoresis techniques for protein and peptide analysis. The authors present cutting-edge techniques for both capillary coatings and analytic detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Areas of special interest covered include combining CE and capillary isoelectric focusing with electrospray mass spectrometry detection to perform proteomic studies and applications in the field of protein-ligand binding. ============== Arabidopsis Protocols Publisher: Humana Pr | ISBN: 0896033910 | edition 1998 | PDF | 414 pages | 22,7 mb Internationally recognized experts offer a comprehensive collection of significant new methods for gene mapping, cloning, and reverse genetics, as well as updated standard protocols for work with Arabidopsis genetics and molecular biology. These time-tested methods range from techniques for the successful growing of Arabidopsis to strategies for gene cloning. The sections on genetics, transformation, and gene expression analysis will be especially helpful to scientists involved in mutant analysis or in producing and analyzing transgenic plants. In many cases, the protocols can also be applied to other plant species, with minor adjustments. Serving both experienced researchers and beginners in the field, Arabidopsis Protocols will immediately become the standard reference for laboratories working with Arabidopsis, the plant of choice to approach many fundamental questions in plant biology. Enzymes of Molecular Biology Publisher: Humana Press | ISBN: 0896032345 | edition 1993 | PDF | 356 pages | 16,3 mb Provides key information on a wide range of enzymes commonly used as tools in molecular biology, helping to minimize the time a scientist spends researching the literature to get reactions to work efficiently and allowing the nonenzymologist to design an experiment. Each chapter gives background information on the enzyme selected and those parameters important in its use, describes both the source and application of the enzyme, and provides details on the size and structure of the protein. Specific parameters essential for achieving an optimized reaction are discussed, along with exemplary practical procedures an protocols. ================== G Protein Signaling: Methods and Protocols Humana Press; 1 edition | September 15, 2003 | ISBN-10: 1588291375 | 264 pages | PDF | 1.23 MB A collection of cutting-edge methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. Among the readily reproducible techniques presented are those for the purification of G proteins and effectors enzymes, assays of these purified G proteins and effector enzymes, and for the study of G proteins interactions with effectors in intact cells. Additional methods are provided for assaying G protein coupled receptor structure, function, and localization; for studying the physiological roles for endogenous G proteins; and for examining lipid and phosphate modifications of RGS proteins. ================== Directed Enzyme Evolution: Screening and Selection Methods Publisher: Humana Press| ISBN: 158829286X | edition 2003 | PDF | 361 pages | 6,7 mb Seasoned practitioners from many leading laboratories describe their best readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. ================== Recombinant Protein Protocols: Detection and Isolation Humana Press; 1 edition | March 5, 1997 | ISBN-10: 089603481X | 468 pages | PDF | 17.17 MB 1st, 1997, Humana Press. Thomas Jefferson University, PA. Comprehensive compendium of step-bystep gene detection and isolation techniques. Companion to: Recombinant Gene Expression Protocols. 67 contributors, 57 U.S. ================== Yac Protocols Humana Press; 1st edition | January 15, 1996 | ISBN-10: 0896033139 | 378 pages | PDF | 14.92 MB In YAC Protocols experienced researchers offer a comprehensive collection of their easily reproducible and proven methods for analyzing complex human, animal, and plant genomes. The step-by-step protocols cover all aspects of yeast artificial chromosomes, ranging from the construction of YAC libraries to their storage, screening, and database management, and to their use in such specialist applications as the transfer of YACs to mammalian cells and the isolation of coding sequences from YACs. YAC Protocols allows workers to add promising approaches adapted from yeast genetics to their personal repertoires and to introduce novel techniques emerging from the accumulating experience with YACs as a tool for genome analysis. Its extensive notes and background information not only significantly enhance the effective use of the methods, but also provide a valuable hands-on resource for those wishing to adapt protocols to suit their own applications. ================== Combinatorial Peptide Library Protocols Humana Press; 1st edition | November 10, 2010 | ISBN-10: 1617370223 | 328 pages | PDF | 11.06 MB A valuable collection of new and unique techniques for the construction and use of peptide libraries. These powerful methods include protocols for the chemical synthesis of peptide libraries, for constructing peptide libraries that are displayed on the surface of filamentous phage or bacteria, and for the rapid screening of these libraries for molecules with biospecific properties. Additional methods permit identifying specific enzyme substrates, investigating the recognition spectra of various binding proteins, epitope mapping, and identifying mimotopes. State-of-the-art techniques, combined with clear step-by-step instructions, make this book an essential tool in the selection of peptides suitable for drug development. Gene Targeting Protocols Publisher: Humana Press | ISBN: 0896033600 | edition 2000 | PDF | 235 pages | 2 mb Presents the latest techniques for gene replacement, gene knockout, and gene repair in animals and plants. Reviews the best gene transfer vehicles, discusses parameters affecting vector uptake and methods for gene targeting. For researchers. DNLM: Gene Targeting laboratory manuals. ============== Calcium-Binding Protein Protocols Volume 1 - 2 Publisher: Humana Press | ISBN: Vol 1 089603688X ISBN: Vol 2 0896036898 | edition 2001 | PDF | 792 pages | 11,5 mb Univ. of Calgary, Alberta, Canada. Features all major biophysical and protein methods to study calcium-binding proteins and includes a detailed discussion of calcium-binding proteins in vitro and in vivo. Many of the methods are also applicable to proteins not binding to calcium.