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DNA Sequencing II: Optimizing Preparation and Cleanup**********************************************
Description
General descriptionKieleczawa′s second volume is devoted to the various methods used for extraction, clean-up, quantification, and analysis of DNA. This volume is divided into four comprehensive sections - DNA Purification, Clean-up of DNA Fragments, Storage of DNA, and Quantifying DNA and RNA - and offers the reader an in-depth presentation of DNA technologies. The text also touches upon the many tools and software programs that are found in a typical modern biology laboratory. This fascinating text is a great addition to your molecular biology library.
Properties
publication infoJ. Kieleczawa, Jones & Bartlett, 2006, 362 pp., hard cover
Table Of Contents
Table of Contents1. Preparation of Difficult DNA Templates using Seven Different Commercial Methods
2. Isolation and Use of Bacterial and P1 Bacteriophage-Derived Artificial Chromosomes
3. Preparation of Genomic DNA from Animal Tissue
4. Extraction of Genomic DNA from Plant Tissue
5. Preparation of cDNA Probes for DNA Microarrays
6. Isolation of Nucleic acids from Paraffin-Embedded Archival Tissues and other "Difficult" Sources
7. Comparison of Sequencing Data for DNAs Prepared Using TempliPhi Technology and Traditional Preparation Protocols
8. Whole Genome Amplification using the Multiple Displacement Amplification Reaction
9. Solid Phase Nucleic Acid Purification using Magnetic Beads
10. The Sanger Institute High Through-put Sequencing Pipeline
11. Comparison of PCR Product Clean-up Methods for High Through-put Capillary Electrophoresis
12. Evaluation of Methods for Clean-up of DNA Sequencing Reactions
13. Thermostable DNA Polymerases for a Wide Spectrum of Applications: Comparison of a Robust Hybrid TopoTaq to other enzymes
14. Prolonged Storage of Plasmid DNAs Under Different Conditions: Effect on Plasmid Integrity Spectral Characteristics and DNA Sequence Quality
15. The DNA Book - A New Method of the Storage and Distribution of Genome Resources
16. Assessing the Quantity, Purity and Integrity of RNA and DNA Following Nucleic Acid Purification
17. Essential Software and Other Tools in Modern Biology Laboratories
