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[SIZE=+4]Practical Streptomyces Genetics
Tobias Kieser, Mervyn J. Bibb, Mark J. Buttner, Keith F. Chater, David A. Hopwood
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, England
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Chapter Titles
1. General introduction to actinomycete biology
2. Growth and preservation of Streptomyces
3. Microscopical methods
4. Specialised biochemical techniques
5. Mutagenesis of Streptomyces by irradiation or chemicals
6. Transposon mutagenesis in Streptomyces
7. In vitro genetic analysis by conjugation and protoplast fusion
8. Preparation and analysis of genomic and plasmid DNA
9. General considerations about gene cloning in Streptomyces
10. Introduction of DNA into Streptomyces
11. Plasmids and their use for gene cloning
12. Streptomyces phages
13. Cloning with phage vectors
14. Gene disruption and gene replacement
15. Reporter systems
16. RNA methods
17. Production and secretion of proteins by Streptomyces
18. Analysing Streptomyces DNA
19. Media, buffers and suppliers
20. Genome maps and genetically marked strains
21. Maps of DNA fragments
22. Maps of plasmids, transposons and phage genomes
Tobias Kieser, Mervyn J. Bibb, Mark J. Buttner, Keith F. Chater, David A. Hopwood
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, England
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[SIZE=+2]"The new manual is terrific"[/SIZE]
This entirely new book replaces the highly successful "Genetic Manipulation of Streptomyces: a Laboratory Manual" of 1985 but is greatly expanded in scope. The five authors lead research teams at the John Innes Centre, Norwich, UK and compilation of the book has also benefited from extensive consultation with the world-wide community of Streptomyces researchers. As well as detailed protocols and hints for all aspects of Streptomyces genetics, including over 100 pages of maps and detailed information on marker genes, it extends into physiology, biochemistry and cytology. It is also a rich source of background information about the biology of this group of mycelial, antibiotic-producing bacteria.Chapter Titles
1. General introduction to actinomycete biology
2. Growth and preservation of Streptomyces
3. Microscopical methods
4. Specialised biochemical techniques
5. Mutagenesis of Streptomyces by irradiation or chemicals
6. Transposon mutagenesis in Streptomyces
7. In vitro genetic analysis by conjugation and protoplast fusion
8. Preparation and analysis of genomic and plasmid DNA
9. General considerations about gene cloning in Streptomyces
10. Introduction of DNA into Streptomyces
11. Plasmids and their use for gene cloning
12. Streptomyces phages
13. Cloning with phage vectors
14. Gene disruption and gene replacement
15. Reporter systems
16. RNA methods
17. Production and secretion of proteins by Streptomyces
18. Analysing Streptomyces DNA
19. Media, buffers and suppliers
20. Genome maps and genetically marked strains
21. Maps of DNA fragments
22. Maps of plasmids, transposons and phage genomes
