Pyrosequencing Protocols (Methods in Molecular Biology)
Pyrosequencing Protocols (Methods in Molecular Biology)
Publisher: Humana Press | ISBN: 1588296458 | edition 2006 | PDF | 230 pages | 3,33 mb
Pyrosequencing Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing, and supplementing each protocol with troubleshooting tips specific to that method.
Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing.
