Antibody Engineering Protocols (Methods in Molecular Biology) (Methods in Molecular Biology)
By Sudhir Paul
Publisher: Humana Press
Number Of Pages: 450
Publication Date: 1995-08-15
ISBN-10 / ASIN: 0896032752
ISBN-13 / EAN: 9780896032750
Binding: Plastic Comb
Product Description:
This comprehensive collection of recently developed methods for producing new antibody reagents by immunization and recombinant DNA techniques contains ready-to-use protocols that illuminate current areas of research on antibody structure, functions, and applications. The methods can be applied in basic immunological studies involving antibody specificity, catalysis, and evolution, and in the isolation of rare antibodies by phage display technology and the engineering of new antibodies by mutagenesis. They offer insight into new ways of developing clinically useful antibody reagents.
Antibody Engineering Protocols constitutes a single-source volume for laboratory investigators who want to minimize extensive literature and methodology searches and to work productively in their fields with reproducible step-by-step protocols.
Antibody Engineering: Methods and Protocols (Methods in Molecular Biology) (Methods in Molecular Biology)
By Benny K. C. Lo
Publisher: Humana Press
Number Of Pages: 576
Publication Date: 2003-12-05
Sales Rank: 832094
ISBN / ASIN: 1588290921
EAN: 9781588290922
Binding: Hardcover
Manufacturer: Humana Press
Studio: Humana Press
Average Rating: 0
Total Reviews: 0
Book Description:
A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.