the molecular weight of substance that has ratio 1 equal 300000 Dalton and this substance is protein which consist of three subunits and each subunit consist of more than 1000 amino acid and only 105 amino acids from the three subunits reacts with the second substance and these amino acids are lysine and hydroxylysine and the molecular weight of substance that has ratio 0.5 equal 192 Dalton and its molecular formula is C8H17N3.HCL