Publisher: Humana Press | ISBN: 089_60392_34 | edition 2001 | PDF | 407 pages | 3,5 mb
This book offers a wide range of readily reproducible methods for sampling, isolating, and culturing all of the major inflammatory cells involved in the pathophysiology of asthma and COPD. The collection of techniques detailed here involve biopsy and sampling of airway liquids such as sputum and exhaled gases such as nitric oxide. Also provided is a full range of methods for the isolation and characterization of cells and for the measurement of the major inflammatory markers, along with protocols for measuring many of the inflammatory mediators and enzymes released during lung inflammation.